Thanks to a longstanding collaboration with Argonne’s Midwest Center for Structural Genomics and the Center for Structural Genomics of Infectious Diseases, the crystal structure of a siderophore binding protein FatB from Desulfitobacterium hafniense has been determined and deposited with the Protein Data Bank (7SF6). This protein was crystallized in the DeNovX laboratories during quantitative crystallization studies and multiple diffraction quality crystals were reproducibly formed on select engineered nucleation features while the control studies produced just a few poorly diffracting crystals. This novel crystallization and structure determination adds to the list of benefits of using DeNovX’s engineered nucleation features that include reproducible improvements in hit percentages, reductions in crystallization onset times, and the production of more crystalline material.